Hotspot

trnK-UUU-rps16

LSC · 4304-5183 · 880 bp

Back to Hamamelis

Pi 0.00227

MarkerSeek score 48.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 3
pi: 0.00227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	AAAAAGGCGCTCAACCTACA	55.0	45.0	57.9	58.0	1069-1070 bp	1.0	79.5
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1008-1009 bp	1.0	78.9
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	1007-1008 bp	1.0	78.9
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	AGAAGGCGGAGGTTTTTACG	55.0	50.0	57.9	58.2	1027-1028 bp	1.0	78.2
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	1023-1024 bp	1.0	78.1