Hotspot

rps3

LSC · 82741-83397 · 657 bp

Back to Haloxylon

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCGAAACAGAAAGACAAGTCCA	GGCGAATAGAACTTTTGTCTGG	40.9	45.5	57.9	57.7	790 bp	1.0	44.3
rps3_p2	CGAAACAGAAAGACAAGTCCAG	GGCGAATAGAACTTTTGTCTGG	45.5	45.5	57.5	57.7	789 bp	1.0	41.7
rps3_p3	CGAAACAGAAAGACAAGTCCA	GGCGAATAGAACTTTTGTCTGG	42.9	45.5	56.4	57.7	789 bp	1.0	40.7
rps3_p4	TCGAAACAGAAAGACAAGTCCA	AGAACTTTTGTCTGGACGTT	40.9	40.0	57.9	55.1	783 bp	1.0	40.2
rps3_p5	TCGAAACAGAAAGACAAGTCCA	GCGAATAGAACTTTTGTCTGGAC	40.9	43.5	57.9	58.0	789 bp	1.0	40.1