Hotspot

psbC

LSC · 33717-35138 · 1422 bp

Back to Haloxylon

Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTGCAGCTGAAGATCCTGAA	GATAGCCGAGCCAGCAAATA	50.0	50.0	57.9	57.8	1632 bp	1.0	77.9
psbC_p2	AAATCCGTGCAGCTGAAGAT	GATAGCCGAGCCAGCAAATA	45.0	50.0	57.9	57.8	1638 bp	1.0	77.7
psbC_p3	TTCGTTTCCCAGGAAATCCG	GATAGCCGAGCCAGCAAATA	50.0	50.0	58.2	57.8	1651 bp	1.0	77.2
psbC_p4	GTGCAGCTGAAGATCCTGAA	GCCGAGCCAGCAAATAAAAA	50.0	45.0	57.9	57.6	1628 bp	1.0	76.3
psbC_p5	AAATCCGTGCAGCTGAAGAT	GCCGAGCCAGCAAATAAAAA	45.0	45.0	57.9	57.6	1634 bp	1.0	76.2