Hotspot

rpl20-rps12

LSC · 68938-69703 · 766 bp

Back to Haloxylon

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
766
variable_sites
1
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CCCTCGAAAGCTTGATCCAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 871 bp 1.0 78.2
rpl20-rps12_p2 CTTGAATGAGCCCCTCGAAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 882 bp 1.0 78.2
rpl20-rps12_p3 TTGAATGAGCCCCTCGAAAG CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 881 bp 1.0 78.2
rpl20-rps12_p4 AGTAAGCCTTGAATGAGCCC CGCCGAGGAACATGTACTAG 50.0 55.0 57.6 57.9 889 bp 1.0 75.7
rpl20-rps12_p5 CCCTCGAAAGCTTGATCCAA GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 870 bp 1.0 75.1