Hotspot

trnP-UGG-psaJ

LSC · 66742-67172 · 431 bp

Back to Haloxylon

Pi 0.00696
MarkerSeek score 46.10
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
431
variable_sites
3
pi
0.00696
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC AGATATGTTTTGAAATCTCGCA 50.0 31.8 58.1 54.2 509 bp 1.0 40.5
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA AGATATGTTTTGAAATCTCGCA 50.0 31.8 58.1 54.2 508 bp 1.0 40.5
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC AGATATGTTTTGAAATCTCGCA 50.0 31.8 58.1 54.2 520 bp 1.0 40.5
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA AGATATGTTTTGAAATCTCGCA 50.0 31.8 58.1 54.2 519 bp 1.0 40.5
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG AGATATGTTTTGAAATCTCGCA 50.0 31.8 58.1 54.2 522 bp 1.0 40.5