Hotspot

atpB-rbcL

LSC · 54726-55498 · 773 bp

Back to Haloxylon

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAGGAAAAGCTACGTCCAG	AGTCTCTGTTTGTGGTGACA	55.0	45.0	58.0	56.6	904 bp	1.0	69.0
atpB-rbcL_p2	GTTTTCAAGTGCGGAAACCC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.2	56.6	845 bp	1.0	67.9
atpB-rbcL_p3	AAAAGCTACGTCCAGTACCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.6	56.6	899 bp	1.0	66.2
atpB-rbcL_p4	CAGGACCCGAAGTAGTAGGA	AGTCTCTGTTTGTGGTGACA	55.0	45.0	57.6	56.6	825 bp	1.0	65.7
atpB-rbcL_p5	GGAGGAAAAGCTACGTCCAG	AGTCTCTGTTTGTGGTGACAT	55.0	42.9	58.0	57.2	904 bp	1.0	65.2