Hotspot

rbcL

LSC · 59297-60724 · 1428 bp

Back to Haloragis

Pi 0.0042

MarkerSeek score 61.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 6
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGTGAAGGGTTTCATTAACGC	TGGGCCGAGTTTAATTGCAA	42.9	45.0	57.1	58.4	1574 bp	1.0	62.0
rbcL_p2	TGTGAAGGGTTTCATTAACGC	TTGGGCCGAGTTTAATTGCA	42.9	45.0	57.1	58.4	1575 bp	1.0	62.0
rbcL_p3	TCGAGTAGACCTTGTTGTTGT	TGGGCCGAGTTTAATTGCAA	42.9	45.0	56.9	58.4	1537 bp	1.0	60.6
rbcL_p4	TCGAGTAGACCTTGTTGTTGT	TTGGGCCGAGTTTAATTGCA	42.9	45.0	56.9	58.4	1538 bp	1.0	60.6
rbcL_p5	CGAGTAGACCTTGTTGTTGTG	TGGGCCGAGTTTAATTGCAA	47.6	45.0	56.9	58.4	1536 bp	1.0	60.3