Hotspot

psbE-petL

LSC · 69085-70322 · 1238 bp

Back to Haloragis

Pi 0.017

MarkerSeek score 73.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 20
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1290-1353 bp	1.0	76.3
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1376-1439 bp	1.0	75.9
psbE-petL_p3	CCCGCAATGAATAGAGAGGG	GTCGTATCTTGCTCAGACCA	55.0	50.0	57.8	57.1	1364-1427 bp	1.0	75.9
psbE-petL_p4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1381-1444 bp	1.0	75.8
psbE-petL_p5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1375-1438 bp	1.0	75.7