Hotspot

trnF-GAA-ndhJ

LSC · 52082-52751 · 670 bp

Back to Haloragis

Pi 0.00302

MarkerSeek score 61.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 2
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	ATAGCCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	810-818 bp	1.0	80.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	768-776 bp	1.0	79.9
trnF-GAA-ndhJ_p3	AGGATGATGCGTCGGGTATA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	827-835 bp	1.0	79.6
trnF-GAA-ndhJ_p4	GGTATAGCCGGGATAGCTCA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.2	58.1	813-821 bp	1.0	79.1
trnF-GAA-ndhJ_p5	ATAGCCGGGATAGCTCAGTT	TCATAATCATCCCCGCCTGA	50.0	50.0	58.0	58.3	850-858 bp	1.0	78.6