Hotspot

rps4-trnT-UGU

LSC · 49181-49489 · 309 bp

Back to Haloragis

Pi 0.0324

MarkerSeek score 68.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 10
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	ACTTCCGACTCTAGGCCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	462-482 bp	1.0	80.2
rps4-trnT-UGU_p2	ACTTCCGACTCTAGGCCTTT	GGTTCGATTCCGATAGTGGG	50.0	55.0	58.1	57.8	421-441 bp	1.0	80.2
rps4-trnT-UGU_p3	ACTTCCGACTCTAGGCCTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	430-450 bp	1.0	79.4
rps4-trnT-UGU_p4	ACTTCCGACTCTAGGCCTTT	GTTCGATTCCGATAGTGGGC	50.0	55.0	58.1	58.5	420-440 bp	1.0	77.4
rps4-trnT-UGU_p5	ACTTCCGACTCTAGGCCTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.1	57.5	470-490 bp	1.0	77.2