Hotspot

trnG-UCC-trnfM-CAU

LSC · 39938-40121 · 184 bp

Back to Haloragis

Pi 0.0223

MarkerSeek score 61.80

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 4
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GGCGTCTCAAGAACGAGAAA	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.2	58.0	311-315 bp	1.0	80.2
trnG-UCC-trnfM-CAU_p2	GGCGTCTCAAGAACGAGAAA	GTTCTTCTTTTCAACGCGGG	50.0	50.0	58.2	58.0	365-369 bp	1.0	79.9
trnG-UCC-trnfM-CAU_p3	ATATGTGTGGGCGTCTCAAG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.7	58.0	320-324 bp	1.0	79.4
trnG-UCC-trnfM-CAU_p4	ATATGTGTGGGCGTCTCAAG	GTTCTTCTTTTCAACGCGGG	50.0	50.0	57.7	58.0	374-378 bp	1.0	79.1
trnG-UCC-trnfM-CAU_p5	GGCGTCTCAAGAACGAGAAA	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.2	57.8	309-313 bp	1.0	79.1