Hotspot

psbZ-trnG-UCC

LSC · 38918-39866 · 949 bp

Back to Haloragis

Pi 0.0312
MarkerSeek score 73.20
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
949
variable_sites
28
pi
0.0312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTGCTTCTCCTGATGGTTGG CGGATAGCGGGAATCGAAC 50.0 57.9 57.8 58.2 1128-1164 bp 1.0 74.4
psbZ-trnG-UCC_p2 TTGCTTCTCCTGATGGTTGG GATAGCGGGAATCGAACCC 50.0 57.9 57.8 57.8 1126-1162 bp 1.0 74.3
psbZ-trnG-UCC_p3 TTGCTTCTCCTGATGGTTGG GGATAGCGGGAATCGAACC 50.0 57.9 57.8 57.8 1127-1163 bp 1.0 74.2
psbZ-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC CGGATAGCGGGAATCGAAC 55.0 57.9 58.3 58.2 1126-1162 bp 1.0 74.0
psbZ-trnG-UCC_p5 GCTTCTCCTGATGGTTGGTC GATAGCGGGAATCGAACCC 55.0 57.9 58.3 57.8 1124-1160 bp 1.0 73.9