Hotspot

trnT-GGU-psbD

LSC · 34119-35646 · 1528 bp

Back to Haloragis

Pi 0.0134

MarkerSeek score 64.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1528
variable_sites: 20
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1684-1715 bp	1.0	81.7
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1647-1678 bp	1.0	80.3
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1692-1723 bp	1.0	79.7
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CGAAACGGTCCCTACGTAAC	50.0	55.0	57.9	58.4	1685-1716 bp	1.0	78.7
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1655-1686 bp	1.0	78.4