Hotspot

trnR-UCU-atpA

LSC · 11458-12002 · 545 bp

Back to Haloragis

Pi 0.0326

MarkerSeek score 61.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 12
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGAATGAAAAGCGTCCATTGT	GAACAGATGGAGCGCTTTCT	42.9	50.0	57.4	58.3	493-666 bp	1.0	70.9
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	ACAGATGGAGCGCTTTCTAC	42.9	50.0	57.4	57.7	491-664 bp	1.0	70.6
trnR-UCU-atpA_p3	GGAATGAAAAGCGTCCATTGT	ATTCAGGAACAGATGGAGCG	42.9	50.0	57.4	57.7	499-672 bp	1.0	70.5
trnR-UCU-atpA_p4	GGAATGAAAAGCGTCCATTGT	GGAGCGCTTTCTACTTCAGG	42.9	55.0	57.4	58.4	485-658 bp	1.0	70.2
trnR-UCU-atpA_p5	GGAATGAAAAGCGTCCATTGT	TGGAGCGCTTTCTACTTCAG	42.9	50.0	57.4	57.6	486-659 bp	1.0	70.1