Hotspot

rps16-trnQ-UUG

LSC · 6487-8071 · 1585 bp

Back to Haloragis

Pi 0.0152

MarkerSeek score 64.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1585
variable_sites: 24
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1658-1725 bp	1.0	79.6
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1669-1736 bp	1.0	79.0
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1679-1746 bp	1.0	77.3
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1680-1747 bp	1.0	77.3
rps16-trnQ-UUG_p5	CCAAGAGCACCTTCATCACT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1724-1791 bp	1.0	76.7