Hotspot

trnK-UUU-rps16

LSC · 4367-5337 · 971 bp

Back to Haloragis

Pi 0.00963

MarkerSeek score 65.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 9
pi: 0.00963
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAGAGGGCGGAGGTTTTTAC	50.0	50.0	58.2	57.8	1050-1065 bp	1.0	80.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAGAGGGCGGAGGTTTTTAC	50.0	50.0	58.2	57.8	1049-1064 bp	1.0	80.5
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	CCCAACCAACAGGAACAGTT	50.0	50.0	58.2	58.2	1082-1097 bp	1.0	80.2
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CCCAACCAACAGGAACAGTT	50.0	50.0	58.2	58.2	1081-1096 bp	1.0	80.2
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	AAGAGGGCGGAGGTTTTTAC	50.0	50.0	58.3	57.8	1065-1080 bp	1.0	79.7