Hotspot

rps18

LSC · 71605-72177 · 573 bp

Back to Halophila

Pi 0.089

MarkerSeek score 76.60

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 573
variable_sites: 42
pi: 0.089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TGCAGGTCTAGGACAGTCTC	CCATGGCTAGAACGAAACGA	55.0	50.0	58.2	58.0	1819-1919 bp	1.0	87.4
rps18_p2	TGCAGGTCTAGGACAGTCTC	TCCATGGCTAGAACGAAACG	55.0	50.0	58.2	58.0	1820-1920 bp	1.0	87.4
rps18_p3	AGACGGAACAGAACCATGTG	CCATGGCTAGAACGAAACGA	50.0	50.0	57.8	58.0	1455 bp	0.5	58.6
rps18_p4	AGACGGAACAGAACCATGTG	TCCATGGCTAGAACGAAACG	50.0	50.0	57.8	58.0	1456 bp	0.5	58.6
rps18_p5	TGCAGGTCTAGGACAGTCTC	AACGCGTTCATTTGCATCAG	55.0	45.0	58.2	58.0	1870 bp	0.5	58.5