Hotspot

clpP

LSC · 73907-74536 · 630 bp

Back to Halophila

Pi 0.137

MarkerSeek score 78.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 630
variable_sites: 86
pi: 0.137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATGGGGAATTGCGTCTCATC	AGCGCGTCAATTGTAGTGAT	50.0	45.0	58.0	58.0	1386 bp	0.5	59.7
clpP_p2	CTCATCCCAAATCAAGGCGA	AGCGCGTCAATTGTAGTGAT	50.0	45.0	58.0	58.0	1372 bp	0.5	59.6
clpP_p3	TCTCATCCCAAATCAAGGCG	AGCGCGTCAATTGTAGTGAT	50.0	45.0	58.0	58.0	1373 bp	0.5	59.6
clpP_p4	TGGTCAGTTCTGAAACGGTG	AGCGCGTCAATTGTAGTGAT	50.0	45.0	58.1	58.0	1683 bp	0.5	59.5
clpP_p5	AACCTTTGCTACCAATGGGG	AGCGCGTCAATTGTAGTGAT	50.0	45.0	58.1	58.0	1400 bp	0.5	59.4