Hotspot

rbcL-accD

LSC · 57950-58788 · 839 bp

Back to Halophila

Pi 0.233

MarkerSeek score 78.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 137
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCTTGTCTCGTTATTGCCGA	CCTTTTGCCCCTCTTTCACT	50.0	50.0	58.0	58.0	2096 bp	0.5	60.0
rbcL-accD_p2	CCTTGTCTCGTTATTGCCGA	TCCTTTTGCCCCTCTTTCAC	50.0	50.0	58.0	58.0	2097 bp	0.5	59.9
rbcL-accD_p3	CAAATGGAGCGCTGAACTTG	CCTTTTGCCCCTCTTTCACT	50.0	50.0	58.0	58.0	1585 bp	0.5	59.8
rbcL-accD_p4	CAAATGGAGCGCTGAACTTG	TCCTTTTGCCCCTCTTTCAC	50.0	50.0	58.0	58.0	1586 bp	0.5	59.7
rbcL-accD_p5	GATTATCCGTGAAGCTGCCA	CCTTTTGCCCCTCTTTCACT	50.0	50.0	58.0	58.0	1603 bp	0.5	59.6