Hotspot

trnQ-UUG-psbK

LSC · 7858-8415 · 558 bp

Back to Halophila

Pi 0.0558

MarkerSeek score 76.70

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 30
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CCCACCCCAATAACGAACTT	CTTACAGCTGCTTGCCAAAC	50.0	50.0	57.8	57.9	1255-1301 bp	1.0	84.8
trnQ-UUG-psbK_p2	AACAAATAGGCCCCAGTTCC	CTTACAGCTGCTTGCCAAAC	50.0	50.0	57.8	57.9	1525-1585 bp	1.0	84.8
trnQ-UUG-psbK_p3	CAAGACCCCACCCCAATAAC	CTTACAGCTGCTTGCCAAAC	55.0	50.0	58.2	57.9	1307 bp	0.5	58.3
trnQ-UUG-psbK_p4	GAAGGATTCGAACCTCCGAG	CTTACAGCTGCTTGCCAAAC	55.0	50.0	57.8	57.9	927 bp	0.5	57.9
trnQ-UUG-psbK_p5	CAAGACCCCACCCCAATAAC	GGTAGAACAAGTCAAGGGGC	55.0	55.0	58.2	58.2	1500 bp	0.5	57.4