Hotspot

trnK-UUU-rps16

LSC · 4011-4774 · 764 bp

Back to Halophila

Pi 0.0476
MarkerSeek score 77.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
764
variable_sites
34
pi
0.0476
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA GAGCAGTACATATGGTCTATTTGA 50.0 37.5 58.3 56.1 819-831 bp 1.0 47.3
trnK-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA AGCAGTACATATGGTCTATTTGA 50.0 34.8 58.3 54.9 818-830 bp 1.0 47.3
trnK-UUU-rps16_p3 CATAGCCGCACTTAAAAGCC GAGCAGTACATATGGTCTATTTGA 50.0 37.5 57.5 56.1 824-836 bp 1.0 47.3
trnK-UUU-rps16_p4 CATAGCCGCACTTAAAAGCC AGCAGTACATATGGTCTATTTGA 50.0 34.8 57.5 54.9 823-835 bp 1.0 47.3
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GAGCAGTACATATGGTCTATTTG 50.0 39.1 58.3 54.7 819-831 bp 1.0 47.3