Hotspot

ycf1

SSC · 122554-127893 · 5340 bp

Back to Halogeton

Pi 0.027

MarkerSeek score 56.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5340
variable_sites: 144
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAAATTCGATTTCGCCCGGT	GGAATCGTCCAACGCGATAT	45.0	50.0	58.0	58.2	981-987 bp	1.0	80.7
ycf1_p2	ATTGCTATCCCAAGCTTCGG	GGAATCGTCCAACGCGATAT	50.0	50.0	58.0	58.2	1298-1304 bp	1.0	80.5
ycf1_p3	CTTTGAACACGCATGGAACC	GGAATCGTCCAACGCGATAT	50.0	50.0	57.9	58.2	1045-1051 bp	1.0	80.4
ycf1_p4	GAACACGCATGGAACCTTTG	GGAATCGTCCAACGCGATAT	50.0	50.0	57.9	58.2	1041-1047 bp	1.0	80.4
ycf1_p5	ACACGCATGGAACCTTTGAT	GGAATCGTCCAACGCGATAT	45.0	50.0	58.1	58.2	1039-1045 bp	1.0	80.1