Hotspot

ccsA-ndhD

SSC · 114123-114302 · 180 bp

Back to Halogeton

Pi 0.0667

MarkerSeek score 74.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 180
variable_sites: 12
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GAAAATTCGGCAATTGTGGC	ACCCGGATTTCGTTCTTTCA	45.0	45.0	56.8	57.4	371-380 bp	1.0	68.8
ccsA-ndhD_p2	GAAAATTCGGCAATTGTGGC	CCCGGATTTCGTTCTTTCAC	45.0	50.0	56.8	57.1	370-379 bp	1.0	66.1
ccsA-ndhD_p3	GAAAATTCGGCAATTGTGGC	CCCGGATTTCGTTCTTTCACT	45.0	47.6	56.8	58.6	370-379 bp	1.0	60.7
ccsA-ndhD_p4	GAAAATTCGGCAATTGTGGC	TACCCGGATTTCGTTCTTTCA	45.0	42.9	56.8	57.3	372-381 bp	1.0	59.8
ccsA-ndhD_p5	GAAAATTCGGCAATTGTGGC	TCAGTTGAGAAAGTGGAAGCT	45.0	42.9	56.8	57.2	348-357 bp	1.0	58.7