Hotspot

rpl32-trnL-UAG

SSC · 112153-112912 · 760 bp

Back to Halogeton

Pi 0.0349

MarkerSeek score 68.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 26
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCCTTTT	TCCGCATAAAAGAGCTGCAT	45.0	45.0	57.9	58.0	1545-1720 bp	1.0	81.8
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCCTTTT	ATCCGCATAAAAGAGCTGCA	45.0	45.0	57.9	58.0	1546-1721 bp	1.0	81.7
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	884-1069 bp	1.0	81.3
rpl32-trnL-UAG_p4	GGCAGCGTTAAAAGCCTTTT	TTGAGATTGGAGAGCGGGTA	45.0	50.0	57.9	58.1	1488-1663 bp	1.0	81.0
rpl32-trnL-UAG_p5	GGCAGCGTTAAAAGCCTTTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.9	57.8	877-1062 bp	1.0	80.8