Hotspot

rrn23-rrn4.5

IRb · 105608-105708 · 101 bp

Back to Halogeton

Pi 0.08

MarkerSeek score 82.40

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 101
variable_sites: 8
pi: 0.08
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	50.0	50.0	58.2	58.0	194-198 bp	1.0	82.9
rrn23-rrn4.5_p2	TAGTAAGCCCACCCCAAGAT	TCACTGCACTTCCACTTGAC	50.0	50.0	57.7	58.0	191-195 bp	1.0	82.8
rrn23-rrn4.5_p3	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	50.0	55.0	58.2	58.0	220-224 bp	1.0	82.3
rrn23-rrn4.5_p4	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	50.0	55.0	58.2	58.1	222-226 bp	1.0	82.2
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	CTGTTAGGATGCCTCAGCTG	50.0	55.0	57.7	58.0	217-221 bp	1.0	82.1