Hotspot

psaJ-rpl33

LSC · 66943-67200 · 258 bp

Back to Halogeton

Pi 0.062

MarkerSeek score 69.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 16
pi: 0.062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TCGCAATTCCAACCGACTAG	45.0	50.0	58.0	58.0	446 bp	1.0	83.3
psaJ-rpl33_p2	TTTTCCCAGATGCGTTGACA	GACAGACACCCAGTTCGATC	45.0	55.0	58.0	58.0	519 bp	1.0	83.0
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	TCGCAATTCCAACCGACTAG	45.0	50.0	58.1	58.0	437 bp	1.0	83.0
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	GACAGACACCCAGTTCGATC	45.0	55.0	58.1	58.0	510 bp	1.0	82.7
psaJ-rpl33_p5	TGTGGCACCGGTACTAAGTA	GACAGACACCCAGTTCGATC	50.0	55.0	58.1	58.0	587 bp	1.0	82.4