Hotspot

psaA-ycf3

LSC · 41181-42032 · 852 bp

Back to Halogeton

Pi 0.0386

MarkerSeek score 71.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 852
variable_sites: 31
pi: 0.0386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGTTTCAGGGCCCTTAGCTA	CGGAAATTGCAGAAGCTTGG	50.0	50.0	58.0	58.0	1084-1100 bp	1.0	85.6
psaA-ycf3_p2	TAGTTTCAGGGCCCTTAGCT	CGGAAATTGCAGAAGCTTGG	50.0	50.0	58.0	58.0	1085-1101 bp	1.0	85.6
psaA-ycf3_p3	GTAGTTTCAGGGCCCTTAGC	CGGAAATTGCAGAAGCTTGG	55.0	50.0	58.0	58.0	1086-1102 bp	1.0	85.6
psaA-ycf3_p4	GGCACTAGGTCCAATGTGAG	CGGAAATTGCAGAAGCTTGG	55.0	50.0	58.0	58.0	1294-1310 bp	1.0	84.8
psaA-ycf3_p5	CCCACTATCGGCCAAACTAC	CGGAAATTGCAGAAGCTTGG	55.0	50.0	58.1	58.0	1317-1333 bp	1.0	84.6