Hotspot

trnG-UCC-trnfM-CAU

LSC · 35825-36032 · 208 bp

Back to Halogeton

Pi 0.0743

MarkerSeek score 77.70

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 15
pi: 0.0743
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GTTCAAATCCTGTCTCCGCA	50.0	50.0	57.8	58.2	273-280 bp	1.0	83.2
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	307-314 bp	1.0	82.3
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	306-313 bp	1.0	82.3
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCAAATCCTGTCTCCGCAAA	50.0	45.0	57.8	57.4	271-278 bp	1.0	80.3
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TTCAAATCCTGTCTCCGCAA	50.0	45.0	57.8	57.4	272-279 bp	1.0	80.3