Hotspot

trnD-GUC-trnY-GUA

LSC · 29773-30144 · 372 bp

Back to Halogeton

Pi 0.036

MarkerSeek score 70.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 13
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	443-507 bp	1.0	77.7
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	442-506 bp	1.0	77.7
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	464-528 bp	1.0	76.7
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	463-527 bp	1.0	76.5
trnD-GUC-trnY-GUA_p5	GCGGTGCTCTAACCAATTGA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.6	58.4	497-561 bp	1.0	75.9