Hotspot

psbM-trnD-GUC

LSC · 28982-29698 · 717 bp

Back to Halogeton

Pi 0.0309

MarkerSeek score 68.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 22
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	40.0	50.0	56.1	58.6	877-1308 bp	1.0	62.3
psbM-trnD-GUC_p2	TTGGCTGACCGTTTTTACAT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.9	58.6	876-1307 bp	1.0	60.5
psbM-trnD-GUC_p3	TTTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.9	58.6	878-1309 bp	1.0	60.4
psbM-trnD-GUC_p4	TGGCTGACCGTTTTTACATAGA	TCAATTGGTTAGAGCACCGC	40.9	50.0	57.7	58.6	875-1306 bp	1.0	59.4
psbM-trnD-GUC_p5	TTTGGCTGACCGTTTTTACAT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.6	58.6	877-1308 bp	1.0	58.7