Hotspot

atpF-atpH

LSC · 12779-13194 · 416 bp

Back to Halogeton

Pi 0.0511

MarkerSeek score 70.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 416
variable_sites: 21
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAATGACCCAAGAAAACG	GTATTGCGAGACAGCCTGAA	50.0	50.0	57.9	58.0	588-593 bp	1.0	83.1
atpF-atpH_p2	CACACACACTCCCTTTCCAA	GTATTGCGAGACAGCCTGAA	50.0	50.0	57.9	58.0	695-700 bp	1.0	82.8
atpF-atpH_p3	TGGCCAATGACCCAAGAAAA	GTATTGCGAGACAGCCTGAA	45.0	50.0	57.9	58.0	590-595 bp	1.0	82.8
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GTATTGCGAGACAGCCTGAA	50.0	50.0	57.9	58.0	614-619 bp	1.0	82.5
atpF-atpH_p5	AGAATACCCTGCTGTGCTTG	GTATTGCGAGACAGCCTGAA	50.0	50.0	57.9	58.0	945-950 bp	1.0	81.8