Hotspot

trnR-UCU-atpA

LSC · 9755-9848 · 94 bp

Back to Halogeton

Pi 0.0455

MarkerSeek score 80.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 94
variable_sites: 4
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAATGAAAAGCGTCCA	ACTGAGGAAGCAGAGACTCT	45.0	50.0	57.9	57.4	251-257 bp	1.0	77.5
trnR-UCU-atpA_p2	TCGGAATGAAAAGCGTCCAT	ACTGAGGAAGCAGAGACTCT	45.0	50.0	57.9	57.4	250-256 bp	1.0	77.5
trnR-UCU-atpA_p3	ATCGGAATGAAAAGCGTCCA	TCAAGAACAGATGGAACGCT	45.0	45.0	57.9	57.2	216-222 bp	1.0	76.1
trnR-UCU-atpA_p4	TCGGAATGAAAAGCGTCCAT	TCAAGAACAGATGGAACGCT	45.0	45.0	57.9	57.2	215-221 bp	1.0	76.1
trnR-UCU-atpA_p5	TCGGAATGAAAAGCGTCCAT	ACTGAGGAAGCAGAGACTCTT	45.0	47.6	57.9	58.1	250-256 bp	1.0	73.4