Hotspot

trnS-GCU-trnG-GCC

LSC · 7902-8762 · 861 bp

Back to Halogeton

Pi 0.049
MarkerSeek score 78.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
861
variable_sites
41
pi
0.049
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CTTTCGTCCACTCAGCCATC AATCAAACCGAAAGACCCCT 55.0 45.0 58.6 57.0 955-962 bp 1.0 72.6
trnS-GCU-trnG-GCC_p2 CTTTCGTCCACTCAGCCATC ATCAAACCGAAAGACCCCTT 55.0 45.0 58.6 57.0 954-961 bp 1.0 72.6
trnS-GCU-trnG-GCC_p3 CTTTCGTCCACTCAGCCATC AATCAAACCGAAAGACCCCTT 55.0 42.9 58.6 57.8 955-962 bp 1.0 70.4
trnS-GCU-trnG-GCC_p4 TTTCGTCCACTCAGCCATCT AATCAAACCGAAAGACCCCT 50.0 45.0 59.0 57.0 954-961 bp 1.0 69.5
trnS-GCU-trnG-GCC_p5 TTTCGTCCACTCAGCCATCT ATCAAACCGAAAGACCCCTT 50.0 45.0 59.0 57.0 953-960 bp 1.0 69.5