Hotspot

trnK-UUU-rps16

LSC · 4159-4765 · 607 bp

Back to Halogeton

Pi 0.0419
MarkerSeek score 74.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
607
variable_sites
25
pi
0.0419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 699-791 bp 1.0 81.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 698-790 bp 1.0 81.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 698-790 bp 1.0 81.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 697-789 bp 1.0 81.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACCGAAACT 50.0 50.0 58.2 57.8 699-791 bp 1.0 81.1