Hotspot

rps11-rpl36

LSC · 79268-79423 · 156 bp

Back to Halimocnemis

Pi 0.0658

MarkerSeek score 80.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 10
pi: 0.0658
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TTTACGTGCACTTTTACGCG	AAAATGTCGACTGATCCGCA	45.0	45.0	57.7	57.9	303-307 bp	1.0	80.7
rps11-rpl36_p2	TACGTGCACTTTTACGCGAA	AAAATGTCGACTGATCCGCA	45.0	45.0	58.5	57.9	301-305 bp	1.0	78.8
rps11-rpl36_p3	TTACGTGCACTTTTACGCGA	AAAATGTCGACTGATCCGCA	45.0	45.0	58.5	57.9	302-306 bp	1.0	78.8
rps11-rpl36_p4	TTTACGTGCACTTTTACGCG	GGGCTTCTGTTCGTCCAATT	45.0	50.0	57.7	58.5	328-332 bp	1.0	77.4
rps11-rpl36_p5	TTTACGTGCACTTTTACGCG	TAAGGGCTTCTGTTCGTCCA	45.0	50.0	57.7	58.7	331-335 bp	1.0	75.9