Hotspot

psbB-psbT

LSC · 73813-73988 · 176 bp

Back to Halimocnemis

Pi 0.0526
MarkerSeek score 74.80
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
176
variable_sites
9
pi
0.0526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 GTAGAATTTGGCGCATTCCA GTTTGAATTTTCGGGGGTTCC 45.0 47.6 56.8 58.0 323-328 bp 1.0 65.4
psbB-psbT_p2 AGTAGAATTTGGCGCATTCCA GTTTGAATTTTCGGGGGTTCC 42.9 47.6 58.3 58.0 324-329 bp 1.0 64.9
psbB-psbT_p3 GTAGAATTTGGCGCATTCCAA GTTTGAATTTTCGGGGGTTCC 42.9 47.6 57.5 58.0 323-328 bp 1.0 63.1
psbB-psbT_p4 AGTAGAATTTGGCGCATTCC GTTTGAATTTTCGGGGGTTCC 45.0 47.6 56.5 58.0 324-329 bp 1.0 63.0
psbB-psbT_p5 GTTCAAGTAGAATTTGGCGCA GTTTGAATTTTCGGGGGTTCC 42.9 47.6 57.4 58.0 329-334 bp 1.0 62.7