Hotspot

psbE-petL

LSC · 64316-65618 · 1303 bp

Back to Halimocnemis

Pi 0.0332

MarkerSeek score 76.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1303
variable_sites: 42
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGAGGTAATAGTTAAAGCCGCT	45.0	45.5	58.0	57.9	1468-1475 bp	1.0	67.7
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCGGAGGTAATAGTTAAAGCC	45.0	45.5	58.0	57.9	1471-1478 bp	1.0	67.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.9	57.9	1460-1467 bp	1.0	67.4
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1457-1464 bp	1.0	67.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.8	57.9	1380-1387 bp	1.0	66.8