Hotspot

trnM-CAU-atpE

LSC · 52152-52416 · 265 bp

Back to Halimocnemis

Pi 0.0711

MarkerSeek score 75.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 18
pi: 0.0711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	AGAGTCATTGGTTCAAATCCA	CTCTCAGACGAGCTAGGACA	38.1	55.0	55.1	58.0	346-357 bp	1.0	55.4
trnM-CAU-atpE_p2	AGAGTCATTGGTTCAAATCCA	ACTCTCAGACGAGCTAGGAC	38.1	55.0	55.1	58.0	347-358 bp	1.0	55.4
trnM-CAU-atpE_p3	AGAGTCATTGGTTCAAATCCA	CGAGCTAGGACACGAGTAGA	38.1	55.0	55.1	58.1	338-349 bp	1.0	55.3
trnM-CAU-atpE_p4	AGAGTCATTGGTTCAAATCCAA	CTCTCAGACGAGCTAGGACA	36.4	55.0	55.9	58.0	346-357 bp	1.0	53.7
trnM-CAU-atpE_p5	AGAGTCATTGGTTCAAATCCAA	ACTCTCAGACGAGCTAGGAC	36.4	55.0	55.9	58.0	347-358 bp	1.0	53.7