Hotspot

trnL-UAA-trnF-GAA

LSC · 47887-48241 · 355 bp

Back to Halimocnemis

Pi 0.0367

MarkerSeek score 66.40

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 13
pi: 0.0367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	445-450 bp	1.0	78.3
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	AAAGATGTGCCAGGAACCAG	50.0	50.0	57.5	58.1	466-471 bp	1.0	77.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	460-465 bp	1.0	77.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	GATGTGCCAGGAACCAGATT	50.0	50.0	57.5	57.9	463-468 bp	1.0	77.5
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	AAAAGATGTGCCAGGAACCA	50.0	45.0	57.5	57.6	467-472 bp	1.0	75.5