Hotspot

trnG-UCC-trnfM-CAU

LSC · 36341-36570 · 230 bp

Back to Halimocnemis

Pi 0.0711

MarkerSeek score 77.60

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 16
pi: 0.0711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GTTCAAATCCTGTCTCCGCA	50.0	50.0	57.8	58.2	295-296 bp	1.0	82.8
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	328-329 bp	1.0	82.0
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	329-330 bp	1.0	81.9
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCAAATCCTGTCTCCGCAAA	50.0	45.0	57.8	57.4	293-294 bp	1.0	79.9
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TTCAAATCCTGTCTCCGCAA	50.0	45.0	57.8	57.4	294-295 bp	1.0	79.9