Hotspot

trnG-GCC-trnR-UCU

LSC · 9549-9671 · 123 bp

Back to Halimocnemis

Pi 0.0678
MarkerSeek score 81.40
Primer available yes
Label trnG-GCC-trnR-UCU

Feature Metadata

label_name
trnG-GCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
123
variable_sites
8
pi
0.0678
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGGAAATAAATTGCGTCCAATAGG 50.0 37.5 58.0 57.9 260-264 bp 1.0 50.6
trnG-GCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGGAAATAAATTGCGTCCAATAGG 55.0 37.5 58.1 57.9 263-267 bp 1.0 50.3
trnG-GCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG TGGAAATAAATTGCGTCCAA 50.0 35.0 58.0 53.8 260-264 bp 1.0 49.8
trnG-GCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG TGGAAATAAATTGCGTCCAA 55.0 35.0 58.1 53.8 263-267 bp 1.0 49.5
trnG-GCC-trnR-UCU_p5 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 219-223 bp 1.0 49.5