Hotspot

ycf1

SSC · 128340-133997 · 5658 bp

Back to Halesia

Pi 0.00848

MarkerSeek score 42.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 48
pi: 0.00848
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAAATTCTGGGAAGAACCA	AAGACTAAGACCGGGGCTAA	40.0	50.0	54.7	57.8	200 bp	1.0	55.1
ycf1_p2	ACAAATTCTGGGAAGAACCA	TTAAGACTAAGACCGGGGCT	40.0	50.0	54.7	57.8	202 bp	1.0	55.1
ycf1_p3	AGTACAAATTCTGGGAAGAACCA	AAGACTAAGACCGGGGCTAA	39.1	50.0	57.7	57.8	203 bp	1.0	54.7
ycf1_p4	TTCTGGGAAGAACCAAAGTT	AAGACTAAGACCGGGGCTAA	40.0	50.0	54.7	57.8	195 bp	1.0	54.7
ycf1_p5	AGTACAAATTCTGGGAAGAACCA	TTAAGACTAAGACCGGGGCT	39.1	50.0	57.7	57.8	205 bp	1.0	54.6