Hotspot

rpl2

IRb · 88323-89811 · 1489 bp

Back to Halesia

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1489
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AACGTGTCACAGCTTACTCC	ATGGGACCTATTATGGGGCA	50.0	50.0	57.8	57.9	1674 bp	1.0	77.7
rpl2_p2	AACGTGTCACAGCTTACTCC	TGGGACCTATTATGGGGCAT	50.0	50.0	57.8	57.9	1673 bp	1.0	77.7
rpl2_p3	AACGTGTCACAGCTTACTCC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	1625 bp	1.0	76.8
rpl2_p4	AACGTGTCACAGCTTACTCC	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	1632 bp	1.0	76.2
rpl2_p5	CGTGTCACAGCTTACTCCTT	TGGGACCTATTATGGGGCAT	50.0	50.0	57.6	57.9	1671 bp	1.0	75.4