Hotspot

matK

LSC · 2065-3588 · 1524 bp

Back to Halesia

Pi 0.00459

MarkerSeek score 61.30

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 7
pi: 0.00459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACGCGGTCTCATAACAAATCA	TGTTTTGACCGTATCGCACT	42.9	45.0	58.0	57.8	1621 bp	1.0	70.8
matK_p2	ACGCGGTCTCATAACAAATCA	CCTTGTTTTGACCGTATCGC	42.9	50.0	58.0	57.5	1624 bp	1.0	67.9
matK_p3	CGCGGTCTCATAACAAATCA	TGTTTTGACCGTATCGCACT	45.0	45.0	56.3	57.8	1620 bp	1.0	65.1
matK_p4	ACGCGGTCTCATAACAAATC	TGTTTTGACCGTATCGCACT	45.0	45.0	56.3	57.8	1621 bp	1.0	65.1
matK_p5	ACGCGGTCTCATAACAAATCA	TTGTTTTGACCGTATCGCAC	42.9	45.0	58.0	57.0	1622 bp	1.0	64.5