Hotspot

ccsA

SSC · 118826-119791 · 966 bp

Back to Halesia

Pi 0.0114

MarkerSeek score 65.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 11
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATGCTATCTTCTAA	TCGCTGGCTTACACGAAATT	50.0	45.0	57.8	58.2	1136 bp	1.0	77.8
ccsA_p2	GGCGGCATGCTATCTTCTAA	AATGGTTCTCGCTGGCTTAC	50.0	50.0	57.8	58.3	1144 bp	1.0	77.3
ccsA_p3	GGCGGCATGCTATCTTCTAA	AAATGGTTCTCGCTGGCTTA	50.0	45.0	57.8	57.5	1145 bp	1.0	75.5
ccsA_p4	GGCGGCATGCTATCTTCTAA	CGCTGGCTTACACGAAATTT	50.0	45.0	57.8	57.4	1135 bp	1.0	74.5
ccsA_p5	GGCGGCATGCTATCTTCTAA	CTCGCTGGCTTACACGAAAT	50.0	50.0	57.8	58.6	1137 bp	1.0	74.3