Hotspot

ndhE-ndhG

SSC · 122490-122708 · 219 bp

Back to Halesia

Pi 0.0228

MarkerSeek score 65.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 5
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGGCTCTAACCATGTTTCGA	ATAGGTGCAATCGCTGTAGC	50.0	50.0	57.6	58.1	340 bp	1.0	77.0
ndhE-ndhG_p2	AGGGCTCTAACCATGTTTCG	ATAGGTGCAATCGCTGTAGC	50.0	50.0	57.6	58.1	341 bp	1.0	76.9
ndhE-ndhG_p3	AACCATGTTTCGACTCGTGA	ATAGGTGCAATCGCTGTAGC	45.0	50.0	57.5	58.1	333 bp	1.0	76.0
ndhE-ndhG_p4	GGGCTCTAACCATGTTTCGA	CAATCGCTGTAGCTCGTCAA	50.0	50.0	57.6	58.4	333 bp	1.0	75.1
ndhE-ndhG_p5	AGGGCTCTAACCATGTTTCG	CAATCGCTGTAGCTCGTCAA	50.0	50.0	57.6	58.4	334 bp	1.0	75.0