Hotspot

rpl32-trnL-UAG

SSC · 117741-118652 · 912 bp

Back to Halesia

Pi 0.0121

MarkerSeek score 56.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 912
variable_sites: 11
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTCTTTCGAGCGGAACTTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1004-1006 bp	1.0	79.8
rpl32-trnL-UAG_p2	CTCTTTCGAGCGGAACTTCA	TTAGAAGATAGCATGCCGCC	50.0	50.0	57.9	57.8	1058-1060 bp	1.0	79.4
rpl32-trnL-UAG_p3	CTCTTTCGAGCGGAACTTCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	997-999 bp	1.0	79.4
rpl32-trnL-UAG_p4	CTCTTTCGAGCGGAACTTCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	1003-1005 bp	1.0	79.3
rpl32-trnL-UAG_p5	CTCTTTCGAGCGGAACTTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	1002-1004 bp	1.0	79.3