Hotspot

ycf4-cemA

LSC · 63934-64889 · 956 bp

Back to Halesia

Pi 0.00963

MarkerSeek score 55.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 9
pi: 0.00963
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	1052-1068 bp	1.0	80.9
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1066-1082 bp	1.0	80.2
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1064-1080 bp	1.0	79.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.1	57.6	1051-1067 bp	1.0	78.3
ycf4-cemA_p5	AGCTGCTGAATTGGCCTATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.6	58.1	1062-1078 bp	1.0	78.0