Hotspot

atpB-rbcL

LSC · 57637-58406 · 770 bp

Back to Halesia

Pi 0.0052

MarkerSeek score 61.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 4
pi: 0.0052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AACGATAAGCCCCGGTTTTT	CACCAGCTTTGAATCCAACAC	45.0	47.6	58.1	58.0	891-899 bp	1.0	71.6
atpB-rbcL_p2	AACGATAAGCCCCGGTTTTT	ACCAGCTTTGAATCCAACACT	45.0	42.9	58.1	58.1	890-898 bp	1.0	71.4
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	929-937 bp	1.0	71.0
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.8	58.1	928-936 bp	1.0	70.8
atpB-rbcL_p5	ATTTGAACGATAAGCCCCGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.3	58.0	896-904 bp	1.0	69.7