Hotspot

trnF-GAA-ndhJ

LSC · 51032-51747 · 716 bp

Back to Halesia

Pi 0.0168

MarkerSeek score 57.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 12
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	822 bp	1.0	80.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	831 bp	1.0	78.0
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.5	58.1	825 bp	1.0	76.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGATAATCATCCGCGCTTGA	50.0	45.0	58.1	57.5	862 bp	1.0	76.5
trnF-GAA-ndhJ_p5	TGGTAGAGCAGAGGACTGAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.4	58.1	845 bp	1.0	75.6